The influence of nanocrystalline gadolinium-doped ceria (Cei_,Gd,O2_y) on the functional status and viability of NCTC clone L929
The hydrodynamic diameter of Ce1.xGdxO2.y nanoparticles in the deionized water (pH 7.2) was 3.2 nm (88.9% in the sample) and 11.1 nm (6.4% in the sample), and Q-potential was about -55 mV, which indicates very high stability of the sol. In the F-12 culture medium, Ce1-xGdxO2-y particle size increased to ~6-7 nm, and a certain number of aggregates ranging from 45 to 60 nm appeared, while their percentage was very small, 1-2%. Nanoparticles of gadolinium-doped ceria in the concentration range of 10-5-10-9 M slightly reduced the level of dehydrogenase activity in the cells after 24 h of cultivation. The use of maximum concentration (10-4 M) resulted in a significant (20%) decrease in the level of dehydrogenase activity compared to the control. Thus, high concentrations of Ce1-xGdxO2-y nanoparticles can cause some toxic effects and affect intracellular metabolism upon their cultivation. Meanwhile, the deviation of the MTT assay result at 10-4 М concentration was not critical, since the administration of the nanoparticles did not lead to an increase in the number of dead cells or to the development of apoptotic processes in the cell culture. Lower concentrations of nanoparticles (10-6-10-9 M) did not cause a toxic effect, but on the contrary, exhibited a stimulating effect, which led to an increase in the number of cells. Cultivation of cells with Ce1-xGdxO2-y nanoparticles in the entire range of concentrations (10-4-10-9 M) did not lead to an increase in the level of lactate dehydrogenase in the culture medium. This confirmed that there was no damage to the cell membranes. We studied the influence of Ce1-xGdxO2-y nanoparticles (10-5-10-9 M) on the levels of intracellular reactive oxygen species and free radicals in the cells. It was shown that in this range of concentrations, gadolinium-doped ceria did not provoke an increase in the levels of intracellular ROS and superoxide anion-radical. Confocal microscopy showed that cerium dioxide nanoparticles penetrated the cells, and their cytoplasmic and lysosomal localization was confirmed. It was shown that the nanoparticles did not penetrate the nucleus, which confirmed the absence of genotoxic effect and DNA damage. Thus, our preliminary analysis allowed concluding that Ce1-xGdxO2-y nanoparti-cles did not possess any acute toxic effect; however, the prospects of their biomedical application can be determined only after a complex study in vitro and in vivo.
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Keywords
нанокристаллический диоксид церия, гадолиний, цитотоксичность, активные формы кислорода, МТТ-тест, nanocrystalline ceria, gadolinium, cytotoxicity, reactive oxygen species, МТТ assayAuthors
| Name | Organization | |
| Popov Anton L. | Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences | antonpopovleonid@gmail.com |
| Tatarnikova Olga G. | Institute of Cell Biophysics of the Russian Academy of Sciences | knopka1606@list.ru |
| Shekunova Taisiya O. | Lomonosov Moscow State University | tasiok@mail.ru |
| Popova Nelli R. | Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences | nellipopovaran@gmail.com |
| Baranchikov Alexander E. | Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences | a.baranchikov@yandex.ru |
| Ivanov Vladimir K. | Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences | van@igic.ras.ru |
| Kozik Vladimir V. | Tomsk State University | vkozik@mail.ru |
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The influence of nanocrystalline gadolinium-doped ceria (Cei_,Gd,O2_y) on the functional status and viability of NCTC clone L929 | Vestnik Tomskogo gosudarstvennogo universiteta. Chimia – Tomsk State University Journal of Chemistry. 2017. № 8. DOI: 10.17223/24135542/8/6
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