Development of spectral techniques for the analysis of biological objects of animal origin

The work is devoted to the elemental analysis of biological objects of animal origin by methods of arc atomic emission spectroscopy and flame photometry with the aim of developing a technique for quantitative chemical analysis. The objects of study were the tissues of internal organs (brain, liver, heart, kidneys), as well as the blood of experimental animals. After the screening analysis, it was shown that the potassium, sodium and phosphorus elements are matrix elements. Moreover, the potassium content by an order of magnitude and more exceeds the content of other elements. Obviously, these elements will significantly influence the definition of regulated impurities. To study the anionic composition of the samples in order to further eliminate the matrix effect, the method of IR-spectroscopy was used. From the conducted studies, it can be assumed that the basis of the ash residue of the brain tissue of experimental rats is K3PO4 with an admixture of sulfates and carbonates. The basis of the ash residue of the cardiac muscle tissue of the experimental rats is K2SO4 with impurities ofphosphates and nitrates. The basis of the ash residue in the experimental rats is KCl and NaCl with impurities of phosphates. The basis of the liver residue of the experimental rats is K2SO4 with carbonate impurities. The basis of the ash residue of the experimental rats is (K/Na)3PO4 with impurities (K/Na)2SO4, (K/Na)2CO3. In the same form, presumably, are all the other elements. Thus, to take into account the matrix effect when conducting analysis of trace elements, it is necessary to strictly calculate the concentration of cation in the analyzed sample and add it in accordance with the anionic composition in the standard samples on a graphite basis. The cation content in the analyzed sample and standards on a graphite basis must strictly comply. To test the effectiveness of this method of taking into account the matrix effect, a standard sample of cod tissue composition was used (MODAS-4 Cormorant Tissue, Poland). The analysis of the standard sample showed that the corrective additive significantly improves the accuracy of the analysis. Student criterion discrepancy in the results is not significant in comparison with random variation. The developed approaches to eliminate matrix effects during spectral analysis by introducing corrective additives form the basis for creating techniques for quantitative chemical analysis of biological objects of animal origin. According to the developed techniques, an analysis of the tissues of experimental rats used to create drugs of rhythm-modulating action was carried out.

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