Monolithic columns for HPLC with sorbent based on 1-vinyl-2-pyrrolidone
Organic monolithic columns have several advantages compared to columns obtained on the basis of inorganic materials. For example, organic monoliths are chemically stable in a wide range of pH values and have the same chemical composition on the surface of the sorbent globules and inside the bulk. Along with classical monolithic sorbents based on divinylbenzene-styrene, substances of different chemical origin can be used for the preparation of monoliths. Most often, these are acrylate-based substances, aromatic monomers with different functional groups or monomers with heteroatoms. The diversity of organic monoliths and the interest in them are related to the fact that chemical features of a stationary phase can have a significant impact on column selectivity. One of the promising monomers for the synthesis of polymeric monolithic materials is 1-vinyl-2 pyrrolidone. This monomer is non-toxic, biocompatible, and also has both hydrophilic and hydrophobic properties. This work describes a novel organic polymer monolithic column was developed by copolymerizing 1-vinyl-2-pyrrolidone, styrene and divinylbenzene. The monolith was synthesized in glass tubes with the inner diameter of 2 mm. The porous structure of the monolithic material was characterized by scanning electron microscopy and low-temperature nitrogen adsorption. Hydrodynamic and chromatographic properties of the prepared columns were studied. To investigate the retention mechanism, a non-polar substance (benzene) and a polar substance (phenol) were used. An examples of small molecules and some polymer molecules separations are presented.
Keywords
1-vinyl-2-pyrrolidone,
porous polymers,
monolithic columns,
HPLC,
1-винил-2 пирролидон,
пористые полимеры,
монолитные колонки,
ВЭЖХAuthors
| Sotnikova Yuliia S. | Novosibirsk State University; Institute of Catalysis named after G.K. Boreskov Siberian Branch of the Russian Academy of Sciences | julias94@catalysis.ru |
| Patrushev Yuri V. | Novosibirsk State University; Institute of Catalysis named after G.K. Boreskov Siberian Branch of the Russian Academy of Sciences | patrush@catalysis.ru |
Всего: 2
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