The effect of polymeric biodegradable materials on the cell-mediated immune response
The effect of monocytic macrophages of human donors on materials based on hydroxyapatite and lactide and glycolide copolymers has been studied. Monocytic macrophages were isolated by magnetic separation from the buffy coats of individual donors and cultured on the surface of materials for 20 days. It was found that secretion of TNF-a, IL-6, and IL-lf by macrophages in presence of materials does not exceed the control level, therefore, it does not cause a chronic inflammatory response. Scaffolds have insignificantly affected the stimulation of TNF-a and IL-1f by macrophages from all the three donors, but secretion of IL-6 gives a donor-specific response. With long-term cultivation, there is a high concentration of IL-8, which can be explained not only by its pro-inflammatory effect, but also by its properties to cause cell migration and promote its adhesion at the sites of material implantation.
Keywords
macrophages,
scaffolds,
inflammation,
response,
cytokines ReferencesAuthors
| Shapovalova Elena G. | Tomsk State University | elenas6691@gmail.com |
| Ivanyuk Elena E. | Tomsk State University; Tomsk National Research Medical Center of the Russian Academy of Sciences | elenakre-mer@yandex.ru |
| Domracheva Lubov V. | Tomsk State University | lvdomracheva42@gmail.com |
Всего: 3
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