APPLICATION OF EXTRACELLULAR CRYOPROTECTANTS FOR OBTAINING ORGAN MATRIX
Purpose: to Assess the impact of extracellular cryoprotectants on the microstructure and composition of the extracellular matrix (ECM) in the perfusion decellularization of whole organs. The experiment was performed on 34 male rats. Prior to performing the perfusion decellularization is advisable expose the sample freeze-thaw cycles with pre-perfusion solution 5% trehalose. It promotes preserve the structure of the matrix at a satisfactory decellularization. The proposed method on a number of estimated is more efficient than conventional analogs.
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Keywords
тканевая инженерия, печень, внеклеточный матрикс, децеллюляризация, замораживание, криопротекторы, tissue engineering, liver, extracellular matrix, decellularization, freezing, cryoprotective agentsAuthors
| Name | Organization | |
| Chernykh A.V. | ||
| Maleev Yu.V. | ||
| Shevtsov A.N. | shan-87@yandex.ru | |
| Pulver A.Yu. | ||
| Leybovich B.Ye. |
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