Repetitive pulsed x-rayand microwaves effect on peroxide level in isolated hepatocytes

The effects of the repetitive pulsed x-ray (8-25 pulse per second pulse repetition rate; 0,003-0,02 mGy/pulse dose) and microwaves (8-25 pps pulse repetition rate, 120-1520 W/cm peak power flux density -PFD) on the peroxide level in isolated mouse hepatocytes using fluorescent probe (H₂DCF-DA) was investigated. The hepatic cells were isolated using the conventional procedure and following the humane treatment guidance. The source of the pulse periodic x-rays is based on the bremsstrahlung of a SINUS-150 accelerator (Russia; 4 ns pulse duration, 300 kV accelerating voltage, 2.5 kA electron current, spectrum of photon energy with maximum 100 kiloelectronvolt). The laboratory generator based on the MI-505 magnetron (Russia, 10 GHz, 100 ns pulse duration) served as a repetitive pulsed microwaves (RPM) source. One time exposure of the RPM or the repetitive pulsed x-ray on the isolated hepatocytes changes the fluorescence strength. It is the evidence of the effect of RPM and repetitive pulsed x-ray on the peroxide production in hepatocytes. The exposure to repetitive pulsed x-ray with the doses of 0,011, 0,02 mGy/pulse and RPM with the peak of PFD of 960, 1520 W/cm and pulse repetition rates of 10-19 pps was the most effective. The condition of the antioxidant system of the irradiated hepatocytes was investigated simultaneously. For this purposes exogenous hydrogen peroxide was added to the irradiated hepatocyte suspension. Exposure of repetitive pulsed x-ray with the dose of 0,02 mGy/pulse and the pulse repetition rate of 13 pps and RPM with the peak of PFD of 960, 1520 W/cm, the pulse repetition rate of 10-19 pps reduced the antioxidant ability of the irradiated cells. The character and intensity of the effect depends on the pulse repetition frequency and the peak power density or dose. The character and intensity of effect can be defined by the rate of ROS generation and utilisation. The pro- and antioxidant elements in different ratio are sensitive to the conditions of exposure. It was shown that the repetitive pulsed microwaves change the peroxide level more in comparison with the pulse periodic x-ray. The increase of peroxide level in the hepatocytes initiated by RPM or repetitive pulsed x-ray leads to the oxidative modification of lipids and proteins that can be a cause of certain biological effects.

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