Interrelation of adhesive and antilysozyme activity of Staphylococcus florae from skin of people with chronic dermatoses

The beginningof any infectious process is an ability of pathogens to attach tissue cells by means of adhesion and then to display all their pathogenicproperties. The structure of normal microflora, its protective properties, ability to interfere with agents adhesion depend on adhesion.Protection against pathogenic microorganisms is caused by lysozyme, which possesses antibacterial action. But in the process of evolutionmicroorganisms learnt to inhibit lysozyme to survive in different human biotopes. Therefore, adhesive ability and microorganismsantilysozyme activity are important biological properties. Studying biological properties of microorganisms of skin microbiocenosispathogens is very topical in cases of some chronic dermatoses, which have obscure etiology. The purpose of this work is to studystaphylococcus floraes interrelation of adhesive and antilysozyme activity from skin of people with chronic dermatoses. Skin washoutsfrom 270 persons with chronic dermatoses aged from 18 till 80 were investigated (psoriasis - 43.1%; eczema - 38.6%; atopic dermatitis- 18.1%). Identification of microorganisms was carried out by standard methods. Adhesive properties were defined by the Brilis method(1986) and antilysozyme activity - by the Bukharin one. The strains were divided into three groups: 1. microorganisms from the affectedskin spots, 2. from healthy skin spots, 3 from skin of persons of the comparison group. S. aureus was determined in 50.4%(759±133 КОЕ/сm2 on the affected skin, 64±26 КОЕ/сm2 on healthy skin). In the 1st group the average index of adhesion (SPA) was2.31±0.23, in the 2nd - 1.8±0.22, in the 3rd - 0.8±0.10 (р <0.05). The SPA for S. aureus of the affected skin was 4.1±0.08. On healthyskin high adhesive isolates were absent. The SPA for affected and healthy spots was at 1.74±0.09 and 1.67±0.07. Isolates with zerovalue of SPA have it at 0.46±0.25 and 0.88±0.12 on the affected and healthy skin accordingly. Staphylococcuss antilysozyme activity(ALA) in the 1st group was 59.1 % of all the studied isolates, in the 2nd group it was 37.5 %, in the 3rd group - 12.6 %. The quantitativevalue of this factor for microorganisms in the first group was 2.7±0.1 (mkg/ml) and in the second group - 2.5±0.2 (mkg/ml). S. aureusisolates showed ALA in 68.9 % of cases in the 1st group, and 56.3 % in the 2nd group. There were no distinctions in S. haemolyticusantilysozyme activity on affected and on healthy skin. ALA of S. aureus isolates was 2.85±0.2 mkg/ml in the 1st group and 2.66±0.2mkg/ml in the 2nd group. Thus, S. aureus isolated from the affected skin has greater intensity of adhesion than isolates from healthyskin. Expressiveness of antilysozyme activity is higher for microorganisms of the affected skin in comparison with healthy one bothquantitatively and qualitatively. The studied pathogenicity properties and their expression frame conditions for S. aureus strains longpersistence.

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