Influence of estimation inoculate of soil microorganisms on some characteristics of oat growth and crop in the conditions of rockdump of Kedrovsky open pit coal mine

The diversity of the conditions and methods of coalmining requires the technology of reclamation of lands disturbed by the operation of mines, open-pit coal mines and benefication plants to beimproved. Research focused on the development of economically feasible methods of restoration of the biologic productivity of the disturbedareas is becoming especially relevant. Methods of accelerating the transformation of soils that were disturbed by industrial operations includethe introduction of soil microorganism formulations capable of growing on lean substrates that are of primary importance for increasing thebioavailability of nutrients to plants. Using the method of inoculation in special agar media microorganisms capable of growing on substratespoor in nutrients were separated from soils  microfungi, microorganisms absorbing mineral nitrogen, and microorganisms capable of decomposingsilicates. Using the method of successive passage of separated microorganisms to liquid nutrient media inocula of microorganisms wereproduced  of microfungi (in 3.B wort), microorganisms absorbing mineral nitrogen (in starch-ammonia medium), and microorganisms capableof decomposing silicates (in Aleksandrov-Zak medium containing aluminium silicates). The original inocula were used to prepare blends ofmicroorganisms in various combinations that were introduced into rock dumps twice per the vegetation period. Oats were sowed on the experimentalplots as a test of bioavailability of nutrients to plants. It was shown empirically that the introduction of the soil microorganism inoculuminto residual soils of the industrial origin stimulates growth and increases the oats yield. Specifically, the experimental plant, as compared to thecontrol group, experienced a larger increase in the plant weight, quicker assimilation of organic material by the plant tissues per leaf area unit(NAR), quantity and weight of grains produced by a plant and the weight of 1,000 grains. Maximum growth and oats yield performance wasidentified in the scenarios with the introduction of the inoculum of microorganisms cultivated in Aleksandrov-Zak medium, both separately andin combination: 1  with microorganisms cultivated in the starch-ammonia medium; 2  with microorganisms cultivated in the starch-ammoniamedium and microfungi.

Keywords

Authors

References