Influence of brassinosteroids on forming protective reactions in rape seedlings under salinity
At present time about one third of all land is affected by salinity. Plants in such conditions have serious physiological disorders. Researchers are looking for ways to prevent the negative effects of salinity on plants. Basic value for understanding the strategy of the survival of plants in extreme conditions has solutions to this problem. Using a hormone showed a decrease in the negative influence of salinity. In our research there was used a plant steroid hormone, distinguished by its physiological activity - epibrassinolide. In the experiment there was studied a negative effect produced by a high concentration of NaCl (175 mM) on the germination of seeds, the accumulation of photosynthetic pigments, the content of proline and the development of rape seedlings under white light. The protective effect of brassinosteroids (10-10 and 10-8 M) was studied. Growth rates of seedlings (the length of the hypocotyl and root), the content of photosynthetic pigments and proline were analyzed for seven days after the beginning of germination under saline conditions. Salinization restricts the growth of plants and reduces their productivity. The most sensitive to the action of chloride salinity under white light was the growth of the root, which was suppressed by 5 times compared with the control. The content of photosynthetic pigments in chloride salinity is reduced by about half. Chloride salinity on rape seedlings responded by a 70-fold increase in proline content. The protective effect of epibrassinolide which, regardless of the analyzed concentration, reduced the negative action of salinization on germination of seeds and growth of the hypocotyl was established. For embryonic roots in saline conditions there appeared the greatest protective effect of EBL in concentration 10 M. When using a high concentration of EBL, a partial restoration of level photosynthetic pigments to the control was shown. Accumulation of proline was decreased by 10 times when using a high concentration of the hormone. This suggests that stress-protective effect of the hormone is due not to a protective effect of proline but its ability to induce alternative protection systems.
Keywords
epibrassinolide,
brassinosteroids,
photosynthetic pigments,
proline,
growth parameters,
эпибрассинолид,
Brassica napus L,
chloride salinization,
seedlings,
брассиностероиды,
пролин,
фотосинтетические пигменты,
ростовые показатели,
Brassica napus L,
хлоридное засоление,
проросткиAuthors
| Efimova Marina V. | Tomsk State University | stevmv555@gmail.com |
| Manuylova Arina V. | Tomsk State University | arinamanuylova@gmail.com |
| Malofiy Мarina К. | Tomsk State University | marina_malofii@mail.ru |
| Kartashov Аlexandr V. | Timirjazev Institute of Plant Physiology of the Russian Academy of Sciences (Moscow) | botanius@yandex.ru |
| Kuznetsov Vladimir V. | Timirjazev Institute of Plant Physiology of the Russian Academy of Sciences (Moscow); Tomsk State University | vlkuzn@ippras.ru |
Всего: 5
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