Study of the population gene pool of the Roman snail (Helix pomatia L.) in urban areas using ISSR-DNA markers | Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya - Tomsk State University Journal of Biology. 2014. № 3 (27).

Study of the population gene pool of the Roman snail (Helix pomatia L.) in urban areas using ISSR-DNA markers

This work is devoted to the study of population structure of Helix pomatia Linnaeus, 1758, which is one of the biggest land snails in Europe. Our objective was to assess the state of five adventitious populations gene pools of this species (248 individuals) living in urbanized areas of the southeastern part of the present areas, basing on the polymerase chain reaction using ISSR DNA markers. We conducted the studies in Belgorod and its surroundings (Russia), as well as in the cities of Kharkov, Kiev and Zhitomir (Ukraine). The analysis was made using primers UBS827 (5'-(AC)₈G-3') and SAS1 (5'-(GTG)₄GC-3'). Amplification was performed in a thermal cycler and MJ Mini MyCycler (Bio-Rad, USA). We found 30 loci of this species (interval from 3 kbp to 200 bp.). The monomorphic and polymorphic amplicons were identified. The degree of genetic variability of populations was determined. According to our study, in most groups quite a high level of heterozygosity was observed (He=0,273±0,150).However, in one of the groups we noted an increase in the proportion of monomorphic loci, caused, probably, by anthropogenic influence. We also established that, despite the strong isolation of the adventitious populations, the degree of genetic differentiation between them was low. According to the model M. Nei (1975) index Gst = 0.166, Nm = 2.519, the analysis of molecular variance (AMOVA) also showed greater similarity between populations of H. pomatia: Ф_л= 0.210, and the average gene flow Nm = 4.1, individual per generation. The obtained data showed a high genetic variability of the studied groups H. pomatia and demonstrate a certain similarity of natural selection vectors in snail populations in urban areas, which is likely to cause certain similar reactions of population gene pools. Nevertheless, anthropogenic impact can reduce genetic diversity in adventitious populations, which can, eventually, lead to the extinction of these groups. The article contains 3 figures, 5 tables, 20 ref.

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Keywords

the urbanized landscape, population gene pool, PCR, terrestrial mollusks, наземные моллюски, популяционный генофонд, урбанизированный ландшафт, ПЦР

Authors

Name	Organization	E-mail
Snegin Eduard A.	Belgorod State National Research University	snegin@bsu.edu.ru
Artemchuk Olesia Y.	Belgorod State National Research University	ris-med@yandex.ru

Всего: 2

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