Established of tobacco transgenic cell culture in vitro with interleukin-18 gene as productionof medicinal human proteins
Established of tobacco transgenic cell culture in vitro with interleukin-18 gene as production of medicinal human proteins. The first to take out of callus and suspension cultures in vitro of of transgenic plants with interleukin-18 human gene of IL18№7-1, IL18№7-11 and IL18№28-3 lines, also no transgenic intact SR1 line. Optimized of а nutritious medium with including of 2,4-D и BAP hormones for these lines cultivation. Established of the cells reduplication time at lines of tobacco transgenic and duration of suspension cells cultivation. Showed that established of callus and suspension culture in vitro of IL18№7-1 and IL18№7-11 lines transgenic tobacco the most of interleukin-18 human proteins actively production
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Authors
| Name | Organization | |
| Karnachuk R.A. | karnach@mail.tsu.ru | |
| Dorofeev V.Yu. | karnach@mail.tsu.ru | |
| Gvozdeva E.S. | karnach@mail.tsu.ru | |
| Medvedeva Yu.V. | karnach@mail.tsu.ru | |
| Ephimova M.V. | karnach@mail.tsu.ru | |
| Churin A.A. | karnach@mail.tsu.ru | |
| Glukhova L.B. | karnach@mail.tsu.ru |
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