EFFECT OF SELECTIVE LIGHT ON ARTEMISIA ANNUA L.CELL CULTURES GROWTH
One of the major source of medicinal substances for human diseases curing is theplants. Artemisia annua L. (sweet wormwood) which are the origin of sesquiterpen lactoneartemisinin and its derivatives with antimalarial, antiparasitic, antiviral and antitumoureffects, is the exercise of such plant.Traditionally artemisinin has been made from intact plants. However, the content ofthis substance in various plant organs is nonuniform. Consequently, for increasing ofsesquiterpene lactones supply on the world markets we must use cell culture technology.The process of creating this technology needed to obtain basic knowledge about thephysiological regulation of biologically active substances content in the plant cell. Selectivelighted controlling of morphogenesis, plant productivity and secondary metabolitesbiosynthesis is the one of the most important factors of this regulation.Thus, the aim of our work was researching a selective light effects on A. annua L.callus and suspension growth parameters.Growth of callus culture A. annua L. for subculture period in 15 days was studied.Maximum values of the growth index were on culture under blue light, while the greenlight suppressed ones. Maximum cell volume were in callus under green light, both redand blue leads to a reduction of this index, this can show increasing of proliferative activityunder blue light.The effect of selective light on A. annua L. suspension growth for 10 days subculturingon NAA and BAP contained medium was studied. There are no significant differencesbetween cell volumes on the selective light and control. Suspension culture under redlight has reduced values of growth index and dry weight production in compare with cultureunder blue light.
Keywords
growth index,
callus culture,
suspension culture,
selective light,
Artemisia annua L.,
индекс роста,
каллусная культура,
суспензионная куль- тура,
селективный свет,
Artemisia annua L.Authors
| Pesyak Sergey V. | Biological Institute of Tomsk State University | taoekk@gmail.com |
Всего: 1
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