The inversion polymorphism of natural populations of malarial mosquito (Anopheles messeae Fall.) in east part of a specific areal

In the present study the cytogenetic investigation of four Anopheles messeae natural populations(two from Krasnoyarsk region, one from Irkutsk region and one from Gusinoozersk inBuryat Republic) in east species areal has been conducted. The two sample species from Krasnoyarskregion have been united in one since they had no statistically significant differences(p<0,05) of frequency of inversion genotypes of all chromosomes. For cytogenetics analysis thelactoacetoorsein preparations of polytenic chromosomes of ovarians nurse cells have beenmade. For cytogenetics diagnostics of species and identification of inversions the standard mapsof polytene chromosomes were used. Statistical significance of differences was estimated bymeans of a Student's t-test and Mann-Whitney U-test by using Statistica 6.0. The results of the researchof four samples presented in the given work have shown the presence of An. beklemisheviand An. messeae. The An. beklemishevi has been only found in samples of Krasnoyarsk, whereeastern boundary of its areal passes. The An. messeae has been found in all samples. In Krasnoyarskpopulations three variants of inversions genotypes: XL00, XL01 и XL11 have been revealed.The XL11 variant prevailed (64%). In Irkutsk and Gusinoozersk populations all individualshave XL11 chromosome genotype. Frequency distribution of inversion genotypes of chromosome2 in four populations was similar: 2R00 were predominating in all populations. The 2R11were not observed. Replacement of alternative homozygotes at 3R in a longitudinal direction wasobserved. In the western populations (Krasnoyarsk) the karyotype 3R11 is absent, whereas in eastpopulation (Gusinoozersk) this variant is presented with 80 % of individuals. 3L00 predominatein Krasnoyarsk and Irkutsk populations, but in Irkutsk a share of 3L01 heterozygote is much more.In population of Gusinoozersk all inversion genotypes of 3L chromosome are presented . The3L11 genotype, which is not found in populations of Krasnoyarsk and Irkutsk, in Gusinoozerskwas 36 %. The analysis of the cytogenetic data has revealed statistical differences of populationsAn. messeae only on frequencies of inversions in the third chromosome. Populations of Irkutskand Gusinoozersk differ on 3R chromosome. Population of Krasnoyarsk differs from Irkutsk andGusinoozersk population on 3L chromosome.

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