Characteristic of antagonistic activity of Staphylococcus aureusin cross-species interaction between microorganisms

The aim of this study is to investigate the antagonistic activity and production of antimicrobialfactors S. aureus in the cross-species interaction.The cross-species interaction in "strain of the antagonist S. aureus - a strainassociates"system was studied by using the original method (patent RU, №2376381)based on testing the biological properties of the investigated antagonist S. aureus, whichwas treated with metabolites and peptidoglycan cell wall of the associative microorganisms.Strain-antagonist S. aureus is isolated from the skin of the hands of the carrier. Allother microorganisms are isolated from female reproductive tract, including strainassociates- Lactobacillus casei, Enterococcus faecalis, S. hominis, Corynebacteriumminutissimum, Enterobacter agglomerans, E. cloacae and Candida albicans, testculture- L. acidophilus, L. casei и S. hominis. The production of antimicrobial substances- lytic enzymes - was studied with the help of traditional biochemical methods.On the example of antagonistic activity of S. aureus it was shown that the biologicalproperties of bacteria may be changed in the cross-species interaction with associativemicroorganisms, which act: indifferently, in stimulating, inhibiting and inverted ways.The factors regulating properties antagonist are the metabolites and fragments of cellwall associates. The ability to regulate the cross-species interaction in the system "prokaryotes- prokaryotes" is new properties fragments of cell walls of bacteria and fungi.The change the antagonism is due to the regulation of production and / or activity of antimicrobialsubstances, such as lysozyme. The received data motivate the position thatantagonistic activity stain - a result of the cross-species interaction between microorganisms,where a stain-antagonist executes the role producent antimicrobial factors, butassociative stains define the possibility and the expression of their production.Thus, associative microorganisms can play an active role in the formation and functioningof microbiocenoses, due to their ability to regulate the biological properties ofbacteria. For example, when transient S. aureus enters a vaginal biotope, the presenceof a Candida sp. will increase the antagonism of staphylococci against indigenous lactobacilli- the predominant microorganisms of the vaginal microbiota, thus contributing tothe colonization of the biotope by staphylococci and the formation of a new microbialcommunity. On the other hand, the decreasing of antagonism S. aureus against lactobacilli,for example, under the action of staphylococci and enterobacteria, would preventthe colonization of the biotope by S. aureus. A new mechanism of colonization resistanceof the microbiocenoses - the reducing colonization properties of transient microorganismsis proposed. Microbial inhibitors of colonization factors of pathogens are prospectivefor the development of new anti-infective drugs.

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