Dynamics of trypsin inhibitor activityin Hedysarum theinum Krasnob. (Fabaceae Lindl.) plant leaves in differentecological and geographical conditions and by mechanical damage

The object of research was Hedysarum theinum Krasnob, a valuable medicinalplant listed in the Red book of the Republic of Altai. The species is spread in the AltaiMountains in a narrow range of ecological conditions: within the limits of a highmountainouszone of vegetation, in surrounding areas of the wood belt on alpine,subalpine and wood meadows as well as on rocky slopes. Earlier it was revealed,that high trypsin inhybitor activity (TIA) in leaves does not allow to recommendthis species as a perspective fodder plant. It can be used either as a fodder additivewith the high maintenance of biologically-active substances or it can be a source ofvegetative raw material with high TIA, intended for being used for medical purposes.Besides, TIA plays an important adaptive role in plants which is studied insufficientlyfor plants of different groups and, furthermore, for rare species. The purpose of thisresearch is to analyze both seasonal and annual dynamics of TIA in leaves of theH. theinum in different ecological and geographical conditions: introduction to theSiberian Botanical Garden TSU (Tomsk), Central Siberian Botanical Garden SBRAS (Novosibirsk) and in Mountain Altai (Ust-Koksinsky district, piedmont Red) andmechanical damage: the estrangement of the leaves at regular intervals - 1 `, 3`, 5`10` and 15 minutes.The analysis of seasonal activity dynamics in the leaves of the plants, cultured inthe Botanical Garden of Tomsk University, showed that the minimum value of this signhad been revealed in a phase of flowering and it was the case within two years ofsupervision. Both wild samples and those cultured in the Central Siberian BotanicalGarden (Novosibirsk) were characterized by identical TIA dynamics with a maximumin a flowering phase. It is established that the reaction of a separate plant to mechanicaldamages is individual. The TIA activity was tended to increase within the first 5 minutesafter mechanical damage of leaves and then, 15 minutes later after the beginning ofthe experience, the return to the level close to initial was a general tendency. It isestablished that a mechanical damage (the estrangement of leaves) is a convenientmodel for studying the role of TIA in the processes of plant adaptation. The change ofthe TIA in the plants of this species during the growing season and mechanical damagereflects a process that is involved in a number of factors of the immune system, whichshould be considered in the process of introduction of plants of this rare species inSiberia to the new conditions, including its practical use.

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