The effect of mutations in genes perception and transmission flare on structure of root hairs of Arabidopsis thaliana (L.) Heynh

An important environmentalfactor that regulates most of the processes ofthe growth and development of the plants A. thaliana is light. At the same time, the role of light during the formation of roots in the root hairs ofplants are still not fully determined. To date, molecular genetic and physiological studies of mutant plants of A. thaliana allowed isolation and sequencing of a number of genes involved in the perception and transmission of the light signal. These include genes RHD3, RHD4, RHD6, SHV3, BST1, PHYA, PHYB, SRS1, GL2, RHL1, RHL2, RHL3, TTG1, WER1 and COW1 The aim of this work was to study the effect of mutant alleles rhd3-1, rhd4-1, rhd6 -1, shv3-1, bst1-1 phyA, phyB, cpc-1, gl-2, rhl1-1, rhl2-1, rhl3-1, ttg-1, wer-1, cow1-1 gene RHD3, RHD4 , RHD6, SHV3, BST1, PHYA, PHYB, SRS1, GL2, RHL1, RHL2, RHL3, TTG1, WER1, COW1 on the structure of root hairs. Plants were grown in aseptic test-tube culture on agar medium Knop, rich in trace elements. When conducting observations of the plants, conventional techniques of vegetation and comparative morphological studies were taken into consideration. The studies found that mutations in the genes of the perception and transmission of the light signal caused changing the morphology of the roots of the hair epiblemy. In plants of all the analyzed mutant lines, roots are very different from the original race Col-0 according to a number of different features: the form of root hairs, their length, the number and degree of branching. The length and number of hairs in plant epiblemy of these mutant strains vary widely. The diameter of the root hairs of plants of the studied mutant lines change less than their length and quantity. Three lines (rhd3-1, rhd4-1 and cow1-1) revealed a change in the parameters of the diameter of the hair epiblemy. Mutant lines rhd3-1 and rhd4-1 differ from wild-type Col-0 cells form root skin outgrowths. The plants of line rhd3-1 form wavy hair epiblemy on the roots, and the plants of line rhd4-1 - bulging with constrictions along the length of root hairs. Mutant strains bst1-1 and cow1-1 are characterized by branched hairs epiblemy. It is shown that the light controls the parameters of the formation of root hairs together with the regulation of many processes ofplant growth and development.