Localization of XL chromosome regions associated with lamin in nurse cells of malaria mosquitoes | Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya - Tomsk State University Journal of Biology. 2013. № 4 (24).

Localization of XL chromosome regions associated with lamin in nurse cells of malaria mosquitoes

Chromosome attachment to the nuclear lamina is one of the main mechanisms of chromosome positioning in the nuclear interior. Previously, contacts of chromosomes with nuclear envelope in semi-squashed malaria mosquitoes nurse cells nuclei were discovered and its species-specificity was showed. We localized nuclear debris on poly-tene chromosomes of nurse cells of sibling species Anopheles atroparvus Thiel. An. messeae Fall. and An. beklemishevi Steg. et Kab. by chromosome immunostaining with using antibodies to fruit fly lamin Dm0. Distribution of lamin on the shortest chromosomes of the set - XL chromosome was analyzed. Lamin was detected in distinct chromosome regions, mainly in interbands. We used only signals, which were found more than in four nuclei. These regions looked like bright bands and were detected in all analyzed specimens. If intra-specific polymorphism by lamin distribution take s place, it cannot be shown by using our methodical approach. However, we found some single nuclei, which had different signals in each homolog chromosomes. The brightest lamin signals quantity, width and their distribution along chromosomes are species-specific features. In An. messeae and An. beklemishevi lamin revealed in chromosome attachment regions, which was mapped during semi-squashed nurse cells nuclei investigation. The lack of lamin signals in XL chromosome attachment region of An. atroparvus is connected with specific morphology of pericentric regions and with limitation of preparation making. We did not observe significant concordance in lamin localization on XL chromosome in analyzed species. However, we found one similarity between An. messeae and An. atroparvus in lamin localization in 2b-2c region. In spite of similar localization, these regions differ from each other by intensity signals, which can reflect differentiation in the strength of chromosomes contacts. The obtained results do not prove data about evolution conservatism of lamin-associated domains localization in distinct cell types. Interspecific differences in lamin distribution along the XL chromosome suggest chromosome region structure transformation, which results in acquisition or loss of the attachment function or modification of the strength of chromosome contact with nuclear envelope.

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Keywords

Anopheles, malaria mosquitoes, nuclear lamina, nuclear envelope, polytene chromosomes, lamin associated domains, chromosome attachment regions to nuclear envelope, nucleus spatial organization, Anopheles, малярийные комары, ядерная ламина, ядерная оболочка, политенные хромосомы, ламин-ассоциированные домены, районы прикрепления хромосом к ядерной оболочке, пространственная организация ядра

Authors

Name	Organization	E-mail
Artemov Gleb N.	Tomsk State University	g-artemov@mail.ru
Sapunov Gleb A.	Tomsk State University	glebsapunov@yandex.ru
Stegniy Vladimir N.	Tomsk State University	gene@res.tsu.ru

Всего: 3

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