Effect of experiment aldesynchronosis on lipid metabolism in rats with obesity

The article presents the results of a study of the effect of desynchronization of circadian rhythms on lipid metabolism in case of obesity. The model of the alimentary obesity was reproduced in 80 outbred male rats with an initial weight of330-380 g by changing the nature of nutrition. The animals were kept in cages for one individual within one month and were at high-calorie diet consisting of standard food (47%), sweet condensed milk (44%), vegetable oil (8%) and vegetable starch (1%) (fats-29.6%, proteins -14.8%, carbohydrates - 55.6%) (diet C11024, Research Dietes, New Brunswick, NJ). To induce desynchronosis, experimental animals were in artificial light or in full darkness for 10 days around the clock. All procedures with animals were performed in accordance with international rules and standards (European Communities Council Directives of 24 November 1986, 86/609/EEC). The study showed that keeping animals at high-calorie diet during 30 days promoted formation of the model of alimentary obesity, as evidenced by a significant increase in their body weight (average 43%). According to biochemical and enzyme-linked immunosorbent analyses of blood serum of rats, diet-induced obesity on the background of the round the clock lighting is accompanied by hypercholesterolemia and hypertriglyceridemia, tendency to hyperglycemia and decreased production of hormones insulin and leptin. Keeping experimental animals with the model of alimentary obesity under long-term continuous darkening, despite their increase in weight, promoted normalization of all the considered parameters. It is assumed that this effect may be conditioned by synthesis of the protective factor during hours of darkness. Thus, to improve the efficiency of obesity treatment, one can recommend shifting the natural light-dark cycle to darkness for non-pharmacological correction of metabolic disorders.

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